Aiming at the problems of unstable production process caused by interface sulfur passivation and unclear kinetics and mechanism of gold dissolution reaction in the process of gold leaching by thiosulfate, starting from metallurgical physical chemistry research, the mesoscopic evolution law of interface gold dissolution structure morphology of gold leaching by thiosulfate in anaerobic conditions was studied, during which the suitable condition for the evolution from slow pitting to fast later-type dissolution of gold will be explored. The mechanism of the electrochemical leaching of gold by thiosulfate and the action of various species will be elucidated. The theoretical basis of thermodynamics and kinetics of gold dissolution will be explored in order to find the control method of the gold dissolution process. Based on the experimental study of sulfur dissolution and its structural evolution, pitting corrosion, and layered dissolution evolution in multi-length scale, the mechanism of interfacial gold dissolution is realized by using quantum chemical calculation combined with reaction transport equation to analyze from the micro atomic level species adsorption structure and charge transfer to mesoscopic dynamic behavior, and then to macro spatiotemporal dynamics, which is close to cyanidation method to realize the large-scale application of thiosulfate interface rapid dissolution of gold.